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human hil 19  (R&D Systems)


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    R&D Systems human hil 19
    Human Hil 19, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hil 19/product/R&D Systems
    Average 93 stars, based on 7 article reviews
    human hil 19 - by Bioz Stars, 2026-02
    93/100 stars

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    Primer pairs used in this study.
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    Primer pairs used in this study.

    Journal: PLoS ONE

    Article Title: Inhibiting Interleukin-19 Activity Ameliorates Esophageal Squamous Cell Carcinoma Progression

    doi: 10.1371/journal.pone.0075254

    Figure Lengend Snippet: Primer pairs used in this study.

    Article Snippet: A cDNA clone coded for the human (h)IL-19 and extracellular domain of hIL-20R1 sequences from was inserted into the expression vector of Pichia pastoris (pPICZ-α; Invitrogen, San Diego, CA, USA).

    Techniques:

    (A) Immunocytochemical staining (mIgG was the negative control) and (B) real-time polymerase chain reaction analysis (HaCaT cells were the positive controls) of the effect of IL-19 and its receptors IL-20R1 and IL-20R2 on CE81T cells. (C) Concentrations of IL-19 in cultured supernatants of CE81T cells were determined at indicated times using ELISA. Data are mean ± SD. All groups: n = 3. *P<0.05 compared with 0 h group. (D) IL-19 increased STAT-3 phosphorylation in CE81T cells, which was attenuated by anti -IL-19 mAb (1BB1). 1BB1 and anti-IL-20R1 mAb (51D) also reduced endogenous STAT-3 phosphorylation in CE81T cells. All experiments were done 3 times and yielded similar results. Data are from a representative experiment.

    Journal: PLoS ONE

    Article Title: Inhibiting Interleukin-19 Activity Ameliorates Esophageal Squamous Cell Carcinoma Progression

    doi: 10.1371/journal.pone.0075254

    Figure Lengend Snippet: (A) Immunocytochemical staining (mIgG was the negative control) and (B) real-time polymerase chain reaction analysis (HaCaT cells were the positive controls) of the effect of IL-19 and its receptors IL-20R1 and IL-20R2 on CE81T cells. (C) Concentrations of IL-19 in cultured supernatants of CE81T cells were determined at indicated times using ELISA. Data are mean ± SD. All groups: n = 3. *P<0.05 compared with 0 h group. (D) IL-19 increased STAT-3 phosphorylation in CE81T cells, which was attenuated by anti -IL-19 mAb (1BB1). 1BB1 and anti-IL-20R1 mAb (51D) also reduced endogenous STAT-3 phosphorylation in CE81T cells. All experiments were done 3 times and yielded similar results. Data are from a representative experiment.

    Article Snippet: A cDNA clone coded for the human (h)IL-19 and extracellular domain of hIL-20R1 sequences from was inserted into the expression vector of Pichia pastoris (pPICZ-α; Invitrogen, San Diego, CA, USA).

    Techniques: Staining, Negative Control, Real-time Polymerase Chain Reaction, Cell Culture, Enzyme-linked Immunosorbent Assay

    (A) CE81T cells treated with human IL-19 (hIL-19) (200 ng/mL or 500 ng/mL, as indicated) and combined with hIL-19 monoclonal antibody (1BB1, 2 ug/mL). (B) CE81T cells treated with hIL-19 (200 ng/ml or 500 ng/mL, as indicated), and combined with anti-IL-20R1 monoclonal antibodies (51D, 2 ug/mL). Proliferation was analyzed using BrdU incorporation assays. IgG was the negative control. Data are mean ± SD. All groups: n = 3. *P<0.05 compared with untreated group, #P<0.05 compared with IL-19 group.

    Journal: PLoS ONE

    Article Title: Inhibiting Interleukin-19 Activity Ameliorates Esophageal Squamous Cell Carcinoma Progression

    doi: 10.1371/journal.pone.0075254

    Figure Lengend Snippet: (A) CE81T cells treated with human IL-19 (hIL-19) (200 ng/mL or 500 ng/mL, as indicated) and combined with hIL-19 monoclonal antibody (1BB1, 2 ug/mL). (B) CE81T cells treated with hIL-19 (200 ng/ml or 500 ng/mL, as indicated), and combined with anti-IL-20R1 monoclonal antibodies (51D, 2 ug/mL). Proliferation was analyzed using BrdU incorporation assays. IgG was the negative control. Data are mean ± SD. All groups: n = 3. *P<0.05 compared with untreated group, #P<0.05 compared with IL-19 group.

    Article Snippet: A cDNA clone coded for the human (h)IL-19 and extracellular domain of hIL-20R1 sequences from was inserted into the expression vector of Pichia pastoris (pPICZ-α; Invitrogen, San Diego, CA, USA).

    Techniques: BrdU Incorporation Assay, Negative Control

    Real-time migration for CE81T cells was monitored using a JuLI smart fluorescent cell analyzer. (A) Motion graph of CE81T cells (count = 5 cells). (B) Quantization of the motion distance (in µm) of CE81T cells (count = 5 cells) (10% FBS was the positive control; IgG, the negative control of anti-IL-19 monoclonal antibody (1BB1) and anti-IL-20R1 monoclonal antibody (51D). All groups: n = 3. *P<0.05 compared with untreated group, #P<0.05 compared with IL-19 group.

    Journal: PLoS ONE

    Article Title: Inhibiting Interleukin-19 Activity Ameliorates Esophageal Squamous Cell Carcinoma Progression

    doi: 10.1371/journal.pone.0075254

    Figure Lengend Snippet: Real-time migration for CE81T cells was monitored using a JuLI smart fluorescent cell analyzer. (A) Motion graph of CE81T cells (count = 5 cells). (B) Quantization of the motion distance (in µm) of CE81T cells (count = 5 cells) (10% FBS was the positive control; IgG, the negative control of anti-IL-19 monoclonal antibody (1BB1) and anti-IL-20R1 monoclonal antibody (51D). All groups: n = 3. *P<0.05 compared with untreated group, #P<0.05 compared with IL-19 group.

    Article Snippet: A cDNA clone coded for the human (h)IL-19 and extracellular domain of hIL-20R1 sequences from was inserted into the expression vector of Pichia pastoris (pPICZ-α; Invitrogen, San Diego, CA, USA).

    Techniques: Migration, Positive Control, Negative Control

    Soft agar colony formation for CE81T cells treated with human IL-19 (hIL-19) (200 ng/ml) or hIL-19 combined with anti-IL-19 mAb (1BB1) and anti-IL-20R1 mAb (51D). (A) Photomicrographs of colonies were taken two weeks after plating. Representative data from three independent experiments are showed. (B) Quantitative analysis of colony formation. IgG was the negative control. All groups: n = 3. Data are mean±SD. *P<0.05 compared with untreated group, #P<0.05 compared with IL-19 group.

    Journal: PLoS ONE

    Article Title: Inhibiting Interleukin-19 Activity Ameliorates Esophageal Squamous Cell Carcinoma Progression

    doi: 10.1371/journal.pone.0075254

    Figure Lengend Snippet: Soft agar colony formation for CE81T cells treated with human IL-19 (hIL-19) (200 ng/ml) or hIL-19 combined with anti-IL-19 mAb (1BB1) and anti-IL-20R1 mAb (51D). (A) Photomicrographs of colonies were taken two weeks after plating. Representative data from three independent experiments are showed. (B) Quantitative analysis of colony formation. IgG was the negative control. All groups: n = 3. Data are mean±SD. *P<0.05 compared with untreated group, #P<0.05 compared with IL-19 group.

    Article Snippet: A cDNA clone coded for the human (h)IL-19 and extracellular domain of hIL-20R1 sequences from was inserted into the expression vector of Pichia pastoris (pPICZ-α; Invitrogen, San Diego, CA, USA).

    Techniques: Negative Control